I built particle-tracking software using MATLAB for a colleague studying biology. The software included a friendly, straightforward user interface that automatically stored and processed all of the users’ experimental data.
It also included a particle tracking algorithm that accurately determined the location of fluorescently labelled proteins in each frame of a video, then tracked the movement of each object throughout the video, and collected/displayed statistics of the particles.
My colleague was studying the movement of motor proteins across microtubule filaments, and so I designed the software to also cross-reference the movement of the particles against an initial bright-field image of the underlying microtubule bundle. In doing so, my software was able to algorithmically determine the path of each motor protein on the surface of each microtubule.
Derek A. Wood 